<<< Full experiment listing

PXD037282-1

PXD037282 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics of Chlamydia muridarum strains in the absence of the conserved tail specific protease
DescriptionThe role of chlamydial proteases in the pathogenesis of Chlamydia spp. has remained largely unknown. In the present study we have generated a Chlamydia muridarum strain that is a null mutant of tail specific protease (TSP). TSP has conserved orthologs in other chlamydial species and structurally similar homologs in multiple free-living and intracellular bacteria. Similar to the roles of its homologs, TSP seems to play a crucial role in chlamydial resistance to elevated temperatures. Importantly, the tsp null mutant is also heavily attenuated in the mouse urogenital tract, indicating that TSP has a crucial role in chlamydial pathogenicity. We have utilized quantitative proteomics to identify chlamydial proteins that have altered abundances in the tsp null mutant compared to wild-type and an isogenic recombinant of the mutant. These proteins indicate potential substrates of this protease and hints toward the molecular function of TSP.
HostingRepositoryMassIVE
AnnounceDate2024-10-24
AnnouncementXMLSubmission_2024-10-24_18:19:45.203.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmma Doud
SpeciesList scientific name: Chlamydia muridarum; common name: agent of mouse pneumonitis; NCBI TaxID: 83560; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListTMT6plex
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-10 12:50:47ID requested
12024-10-24 18:19:45announced
Publication List
no publication
Keyword List
submitter keyword: chlamydia, tail specific protease, LC-MS/MS
Contact List
David E. Nelson
contact affiliationIndiana University School of Medicine
contact emailnelsonde@indiana.edu
lab head
Amber L. Mosley
contact affiliationIndiana University School of Medicine
contact emailalmosley@iu.edu
lab head
Emma Doud
contact affiliationIUSM
contact emailedoud@iu.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000090509/