PXD037282-1
PXD037282 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative proteomics of Chlamydia muridarum strains in the absence of the conserved tail specific protease |
| Description | The role of chlamydial proteases in the pathogenesis of Chlamydia spp. has remained largely unknown. In the present study we have generated a Chlamydia muridarum strain that is a null mutant of tail specific protease (TSP). TSP has conserved orthologs in other chlamydial species and structurally similar homologs in multiple free-living and intracellular bacteria. Similar to the roles of its homologs, TSP seems to play a crucial role in chlamydial resistance to elevated temperatures. Importantly, the tsp null mutant is also heavily attenuated in the mouse urogenital tract, indicating that TSP has a crucial role in chlamydial pathogenicity. We have utilized quantitative proteomics to identify chlamydial proteins that have altered abundances in the tsp null mutant compared to wild-type and an isogenic recombinant of the mutant. These proteins indicate potential substrates of this protease and hints toward the molecular function of TSP. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-10-24 |
| AnnouncementXML | Submission_2024-10-24_18:19:45.203.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Chlamydia muridarum; common name: agent of mouse pneumonitis; NCBI TaxID: 83560; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | TMT6plex |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-10-10 12:50:47 | ID requested | |
| ⏵ 1 | 2024-10-24 18:19:45 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: chlamydia, tail specific protease, LC-MS/MS |
Contact List
| David E. Nelson | |
|---|---|
| contact affiliation | Indiana University School of Medicine |
| contact email | nelsonde@indiana.edu |
| lab head | |
| Amber L. Mosley | |
| contact affiliation | Indiana University School of Medicine |
| contact email | almosley@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000090509/ |
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