PXD037256-1

PXD037256 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of H460 and MCF7 cell lines grown in 2D and 3D culture conditions
Description	In this study we analyzed the proteomic profiles of H460, and MCF7 3D tumor spheroids grown either in standard medium (3D_SM), or in FBSlow conditions (3D_FBSlow) and compared them to their relative 2D cultures (2D). Standard medium consisted of DMEM/F-12 supplemented with 0.5% Glucose, 2.5mM L-Glutamine, 2% B-27, 5μg/ml Heparin, 20μg/ml Insulin, 20ng/ml EGF, 20ng/ml Recombinant Human bFGF, 0.1% Bovine Serum Albumin (BSA) and 1% (v/v) of penicillin/streptomycin 100U/ml. FBSlow medium consisted of customized nutrient-restricted RPMI or DMEM culture media supplemented with only 2% FBS.
HostingRepository	PRIDE
AnnounceDate	2025-01-20
AnnouncementXML	Submission_2025-01-20_05:13:11.626.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Gaspari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 07:47:47	ID requested
⏵ 1	2025-01-20 05:13:12	announced

10.1186/S13046-023-02641-0;

submitter keyword: lung cancer, breast cancer, tumor spheroids, glucose metabolism, metastasis

Marco Gaspari
contact affiliation	Department of Experimental and Clinical Medicine, University of Catanzaro
contact email	gaspari@unicz.it
lab head
Marco Gaspari
contact affiliation	University of Catanzaro
contact email	gaspari@unicz.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037256
     1. Label: PRIDE project
     2. Name: Proteomic analysis of H460 and MCF7 cell lines grown in 2D and 3D culture conditions