PXD037233 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Engineered live bacteria suppress Pseudomonas aeruginosa lung infection in mouse and dissolve biofilm in endotracheal tubes from patients |
| Description | Engineered live bacteria could provide a new modality for treating lung infections , a major cause of mortality worldwide. Here, we engineered a genome-reduced human lung bacterium, Mycoplasma pneumoniae, to treat ventilator-associated pneumonia (VAP), a disease with high hospital mortality when associated with Pseudomonas aeruginosa biofilms. After validating the biosafety of an attenuated M. pneumoniae chassis in mice, we introduced four transgenes in the chromosome by transposition, to implement bactericidal and biofilm degradation activities. We show that this engineered strain has high efficacy against an acute P. aeruginosa lung infection in a mouse model. In addition, we demonstrate that the engineered strain can dissolve biofilms formed in endotracheal tubes of VAP patients and can be combined with antibiotics targeting the peptidoglycan layer to increase efficacy against gram-positive and gram-negative bacteria. We expect that our |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_09:07:01.818.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rocco Mazzolini |
| SpeciesList | scientific name: Mycoplasma pneumoniae (strain ATCC 29342 / M129); NCBI TaxID: 272634; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-10-10 07:14:45 | ID requested | |
| 1 | 2023-10-24 11:03:17 | announced | |
| ⏵ 2 | 2023-11-14 09:07:06 | announced | 2023-11-14: Updated project metadata. |
Publication List
| Mazzolini R, Rodr, í, guez-Arce I, Fern, á, ndez-Barat L, Pi, ñ, ero-Lambea C, Garrido V, Rebollada-Merino A, Motos A, Torres A, Grill, ó MJ, Serrano L, Lluch-Senar M, Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms. Nat Biotechnol, 41(8):1089-1098(2023) [pubmed] |
Keyword List
| submitter keyword: Engineered live bacteria |
| Pseudomonas aeruginosa |
| lung infection |
| mouse |
| biofilm |
| endotracheal tubes |
Contact List
| Maria Lluch-Senar |
|---|
| contact affiliation | Pulmobiotics, LTD |
| contact email | maria.lluch@pulmobio.com |
| lab head | |
| Rocco Mazzolini |
|---|
| contact affiliation | Pulmobiotics ltd |
| contact email | rocco.mazzolini@pulmobio.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037233 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037233
- Label: PRIDE project
- Name: Engineered live bacteria suppress Pseudomonas aeruginosa lung infection in mouse and dissolve biofilm in endotracheal tubes from patients
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