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PXD037203-1

PXD037203 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNewly synthesized glycoproteins during ex vivo lung perfusion
DescriptionProteins that were newly-synthesized during ex vivo lung perfusion were labeled with an azido-sugar Ac4GalNAz in the perfusate. These proteins were enriched using click chemistry to attach an alkyne-desthiobiotin group to the azido-labeled proteins, then pulled out of solution using streptavidin beads. The overall goal is to better understand the effects of warm ischemia injury in lungs destined for lung transplantation.
HostingRepositoryMassIVE
AnnounceDate2023-05-04
AnnouncementXMLSubmission_2023-05-04_07:46:26.390.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrian Frey
SpeciesList scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116;
ModificationListAcetyl; Methyl; Phospho; Carbamidomethyl; Oxidation; Deamidated; L-citrulline; Formyl; Glu->pyro-Glu; Dehydrated; Butyryl; Dimethyl; Trimethyl; Carboxy; HexNAc
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-07 11:54:27ID requested
12023-05-04 07:46:26announced
Publication List
no publication
Keyword List
submitter keyword: Lung Proteomics, Ex Vivo Lung Perfusion, Lung Transplantation, Warm Ischemia Injury, Azido Sugar, GalNAz, Click Chemistry, Newly Synthesized Proteins
Contact List
Brian L. Frey
contact affiliationUniversity of Wisconsin - Madison
contact emailbfrey@chem.wisc.edu
lab head
Brian Frey
contact affiliationUniversity of Wisconsin Madison
contact emailbfrey@chem.wisc.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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