PXD037198-1

PXD037198 is an original dataset announced via ProteomeXchange.

Title	Analysis of the effect of ClpC1 directed antibiotics on ClpC1, ClpC2 and ClpC3 protein levels.
Description	Ecumicin and Cyclomarin A (CymA) are known antimicrobial compounds that target the NTD of ClpC1. In proteomics analysis of compound treated M. smegmatis, two novel protein, ClpC2 and ClpC3, have been identified as strong interactors of those compounds in addition to ClpC1. In this study we used PRM to monitor the levels of ClpC1, ClpC2 and ClpC3 over time after treatment with CymA or ecumicin or CymA-based BacPROTACs.
HostingRepository	PanoramaPublic
AnnounceDate	2023-06-07
AnnouncementXML	Submission_2023-06-07_14:51:42.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Hoi
SpeciesList	scientific name: Mycolicibacterium smegmatis; NCBI TaxID: 1772;
ModificationList	Carbamidomethyl; Oxidation; Label:13C(6)15N(4)
Instrument	Q Exactive HF-X; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-06 20:50:37	ID requested
⏵ 1	2023-06-07 14:51:42	announced

Hoi DM, Junker S, Junk L, Schwechel K, Fischel K, Podlesainski D, Hawkins PME, van Geelen L, Kaschani F, Leodolter J, Morreale FE, Kleine S, Guha S, Rumpel K, Schmiedel VM, Weinstabl H, Meinhart A, Payne RJ, Kaiser M, Hartl M, Boehmelt G, Kazmaier U, Kalscheuer R, Clausen T, Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival. Cell, 186(10):2176-2192.e22(2023) [pubmed]

submitter keyword: BacPROTAC, PROTAC, protein degradation, TPD, antibiotics

Markus Hartl
contact affiliation	Max Perutz Labs
contact email	markus.hartl@univie.ac.at
lab head
David Hoi
contact affiliation	Max Perutz Labs, Institute of Molecular Pathology
contact email	david.hoi@imp.ac.at
dataset submitter

Panorama Public dataset URI