PXD037183-1

PXD037183 is an original dataset announced via ProteomeXchange.

Title	The structural basis for the self-inhibition of DNA binding by apo-σ70 - CLMS data
Description	Performing crosslinking mass spectrometry and intergrative structural modelling to elucidate, for the first time, structure models of apo-σ70. Conclusively, we provide structural information to show that most of the DNA-binding capabilities of apo-σ70 are conformationally-inhibited and can be activated mostly in the context of transcription. CL-MS performed in E. coli detects crosslinks unique to the compact fold of apo-σ70 occurring at stationary growth phase.
HostingRepository	PRIDE
AnnounceDate	2022-10-13
AnnouncementXML	Submission_2022-10-13_02:34:44.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KhalilJoron
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-05 00:49:41	ID requested
⏵ 1	2022-10-13 02:34:44	announced

Dataset with its publication pending

submitter keyword: σ70, transcription, sigma70, RpoD

EitanLerner
contact affiliation	Biological chemistry department, Alexander Silberman Institute Of Life Sciences, Hebrew University
contact email	eitan.lerner@mail.huji.ac.il
lab head
KhalilJoron
contact affiliation	Hebrew University of Jerusalem
contact email	khalil.joron@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037183

PRIDE project URI

• PRIDE
  1. PXD037183
     1. Label: PRIDE project
     2. Name: The structural basis for the self-inhibition of DNA binding by apo-σ70 - CLMS data