PXD037155-1

PXD037155 is an original dataset announced via ProteomeXchange.

Title	Lineage-specific oncogenes drive growth of major forms of human cancer using common downstream mechanisms
Description	Aim of the experiment was to identify changes in protein-protein interactions between proliferating and non-proliferating cells. For this purpose, RKO cells were treated with 20nM Trametinib, HCT116 with 8nM Trametinib, and NCI-H1975 100nM Osimertinib. At these concentrations, the wild type cell lines experience G1 cell cycle arrest, whereas the corresponding PTEN knockout cell lines continue to proliferate. PISA was performed at 2h time point, before the changes in gene expression and cell cycle distribution took place in the matching single-cell transcriptomics analysis, and at 24h where cell cycle arrest had occurred.
HostingRepository	PRIDE
AnnounceDate	2025-07-17
AnnouncementXML	Submission_2025-07-17_14:04:04.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Massimiliano Gaetani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-03 11:20:38	ID requested
⏵ 1	2025-07-17 14:04:05	announced

Dataset with its publication pending

submitter keyword: proteomics,PISA assay, protein solubility, lung cancer, proliferation, LC-MS/MS, colorectal cancer

Massimiliano Gaetani
contact affiliation	Chemical Proteomics core facility Department of Medical Biochemistry and Biophysics (MBB) Karolinska Institutet, Sweden
contact email	massimiliano.gaetani@ki.se
lab head
Massimiliano Gaetani
contact affiliation	Karolinska Institutet
contact email	massimiliano.gaetani@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD037155

PRIDE project URI

• PRIDE
  1. PXD037155
     1. Label: PRIDE project
     2. Name: Lineage-specific oncogenes drive growth of major forms of human cancer using common downstream mechanisms