PXD037151-1

PXD037151 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HDX-MS of NicA2 and a NicA2 variant with increased activity
Description	Here we have used HDX-MS to investigate the change in structure/dynamics in NicA2 and a variant with increased activity (v321) that was generated using directed evolution
HostingRepository	PRIDE
AnnounceDate	2023-11-16
AnnouncementXML	Submission_2023-11-16_01:15:06.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Calabrese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-03 11:19:27	ID requested
⏵ 1	2023-11-16 01:15:07	announced

Publication List

Dulchavsky M, Mitra R, Wu K, Li J, Boer K, Liu X, Zhang Z, Vasquez C, Clark CT, Funckes K, Shankar K, Bonnet-Zahedi S, Siddiq M, Sepulveda Y, Suhandynata RT, Momper JD, Calabrese AN, George O, Stull F, Bardwell JCA, Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme. Nat Chem Biol, 19(11):1406-1414(2023) [pubmed]

Dulchavsky M, Mitra R, Wu K, Li J, Boer K, Liu X, Zhang Z, Vasquez C, Clark CT, Funckes K, Shankar K, Bonnet-Zahedi S, Siddiq M, Sepulveda Y, Suhandynata RT, Momper JD, Calabrese AN, George O, Stull F, Bardwell JCA, Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme. Nat Chem Biol, 19(11):1406-1414(2023) [pubmed]

Keyword List

submitter keyword: NicA2, directed evolution, nicotine oxidoreductase

submitter keyword: NicA2, directed evolution, nicotine oxidoreductase

Contact List

Antonio Calabrese
contact affiliation	Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom, LS2 9JT
contact email	a.calabrese@leeds.ac.uk
lab head
Antonio Calabrese
contact affiliation	School of Molecular and Cellular Biology, University of Leeds
contact email	a.calabrese@leeds.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD037151
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD037151

PRIDE project URI

Repository Record List

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