PXD037146-1

PXD037146 is an original dataset announced via ProteomeXchange.

Title	Human - Cells and Extracellular vesicles of Hela and MNT-1 WT vs CD63 KO Proteomes 1
Description	CD63 is a tetraspanin, which organize on cholesterol enriched microdomains, especially on late endosomes. Previous work of our team has shown that CD63 could regulate the sorting of cargoes into ILVs through an ESCRT-independent manner in melanocytes. However, CD63 has also been involved in other trafficking events in other cell type and so far, we have no clear idea of the general process that CD63 would control and would unify these disparate reports. In order to elucidate to general role of CD63 we compared two cell lines, Hela and MNT-1 that we Knock-out for CD63 by CRISPR in both cell lines and investigated the EV generation and the expression level of proteins involved in this process in cell lysates and the EV pellet obtained by SEC.
HostingRepository	PRIDE
AnnounceDate	2024-04-19
AnnouncementXML	Submission_2024-04-19_03:48:58.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-03 11:04:54	ID requested
⏵ 1	2024-04-19 03:48:59	announced
2	2024-10-22 06:36:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: CD63, Extracellular vesicles (EV), Hela, MNT-1, Cell lines

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD037146

PRIDE project URI

• PRIDE
  1. PXD037146
     1. Label: PRIDE project
     2. Name: Human - Cells and Extracellular vesicles of Hela and MNT-1 WT vs CD63 KO Proteomes 1