PXD037123-1

PXD037123 is an original dataset announced via ProteomeXchange.

Title	The Scar/WAVE complex drives normal actin protrusions without the VCA domain, but proline-rich domains are required
Description	The Scar/WAVE regulatory complex (WRC) is the main catalyst of pseudopod and lamellipodium formation during cell migration. Its actin nucleation activity has been attributed to its ability to activate the Arp2/3 complex through the VCA domain of Scar/WAVE. Here we show in both B16-F1 mouse melanoma cells and Dictyostelium discoideum cells that the WRC with its VCA domain deleted can still induce the formation of morphologically normal actin protrusions. Its function is lost only after further deletion of Scar/WAVE’s (in Dictyostelium cells) or both Abi and WAVE’s (in B16-F1 cells) proline-rich domains. Moreover, mass spectrometry analysis identified BAIAP2 and Vasp (both promotors of actin polymerization) as specific interactors of WRC polyproline domains. Thus we conclude that proline-rich domains play a central role in actin nucleation by concentrating other effectors needed to form actin protrusions. Together, these findings suggest a new mechanism for WRC action independent of the VCA-Arp2/3 interaction.
HostingRepository	PRIDE
AnnounceDate	2024-08-30
AnnouncementXML	Submission_2024-08-30_04:10:07.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Lilla
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 04:39:16	ID requested
⏵ 1	2024-08-30 04:10:07	announced
2	2024-10-22 06:57:33	announced	2024-10-22: Updated project metadata.

10.1016/J.CUB.2024.08.013;

submitter keyword: Polyproline., Scar/WAVE,Actin, WRC, Arp2/3

Sara Rossana Zanivan
contact affiliation	CRUK - Beatson Institute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Sergio Lilla
contact affiliation	Proteomics
contact email	s.lilla@beatson.gla.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037123
     1. Label: PRIDE project
     2. Name: The Scar/WAVE complex drives normal actin protrusions without the VCA domain, but proline-rich domains are required