PXD037102-1

PXD037102 is an original dataset announced via ProteomeXchange.

Title	LC-MS characterization of anti-PD-L1 and CXCR4 bispecific nanobodies
Description	Antibodies and derivative drugs targeting immune checkpoints have been approved for the treatment of several malignancies, but there are fewer responses in patients with pancreatic cancer. Here, we designed a nanobody molecule with bi-targeting on PD-L1 and CXCR4, as both targets are overexpressed in many cancer cells and play important roles in tumorigenesis. The nanobody sequences targeting PD-L1 and CXCR4 were linked by the (G4S)3 flexible peptide to construct the anti-PD-L1/CXCR4 bispecific nanobody. The bispecific nanobody was expressed in E. coli cells and purified by affinity chromatography. The purified nanobody was biochemically characterized by mass spectrometry, Western blotting and flow cytometry to confirm the molecule and its association with both PD-L1 and CXCR4. The biological function of the nanobody and its anti-tumour effects were examined.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:34:07.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shuyi xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 04:01:47	ID requested
⏵ 1	2024-10-08 11:34:08	announced

Hao S, Xu S, Li L, Li Y, Zhao M, Chen J, Zhu S, Xie Y, Jiang H, Zhu J, Wu M, Tumour inhibitory activity on pancreatic cancer by bispecific nanobody targeting PD-L1 and CXCR4. BMC Cancer, 22(1):1092(2022) [pubmed]

10.1186/s12885-022-10165-7;

submitter keyword: Nanobody, LC-MS,Bispecific, protein,complete MW

Mingyuan Wu
contact affiliation	School of Pharmacy, Engineering Research Center of Cell& Therapeutic Antibody, Ministry of Education,Shanghai Jiao Tong University
contact email	Mingyuanwu@sjtu.edu.cn
lab head
shuyi xu
contact affiliation	Shanghai Jiao Tong University
contact email	shuyixu16@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037102

PRIDE project URI

• PRIDE
  1. PXD037102
     1. Label: PRIDE project
     2. Name: LC-MS characterization of anti-PD-L1 and CXCR4 bispecific nanobodies