PXD037062-1

PXD037062 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of the membrane vesicles in Pseudomonas aeruginosa under X-ray irradiation
Description	Pseudomonas aeruginosa, X-ray irradiation, inner membrane blebbing, membrane vesicles Almost all bacteria produce membrane vesicles (MVs) that have been found to be generally composed of lipopolysaccharides (LPSs), phospholipids (PLs), outer membrane proteins, periplasmic proteins and peptidoglycan (PG), cytoplasmic proteins, as well as nucleic acids, metabolites and signaling molecules. We have recently demonstrated that X-ray irradiation strongly induced the MV production in P. aeruginosa, but the detailed biogenesis mechanisms and protein components of MVs were largely unclear. In this study, we performed a quantitative proteomics analysis of the MVs; a total of 2320 proteins were identified, among which 72.33% were cytoplasmic proteins, followed by periplasmic proteins (12.59%), IMPs (8.45%), OMPs (5.26%) and others (1.38%). Gene ontology (GO) enrichment analysis of biological processes showed that nucleoside phosphate metabolic process, ribose phosphate metabolic process, nucleoside metabolic process, and purine-containing compound biosynthetic process were significantly enriched. As expected, cellular component analysis confirmed the enrichment of cytoplasm. Notably, molecular functional enrichment analysis revealed that a large proportion of proteins that were related to RNA binding, tRNA binding, RNA methyltransferase activity, aminoacyl-tRNA ligase activity, and ligase activity were highly enriched. KEGG enrichment analysis showed that pathways involved in ribosome, purine metabolism, tRNA biogenesis and RNA degradation were enriched. These results suggest that under X-ray irradiation stress, P. aeruginosa PAO1 cells could transport the damaged nucleic acids by producing MVs, and the RNA binding and degradation proteins contained in MVs may help P. aeruginosa to identify and degrade damaged RNA.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:02:21.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenling Wang
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-28 01:01:01	ID requested
⏵ 1	2025-08-24 16:02:22	announced

Zhang L, Shen Z, Chen Y, Ma C, Huang M, He Y, Wang G, Huang D, Su B, Jiang B, Luo Y, Li W, Lian M, Xu X, Cheng X, Wang Z, X-Ray Irradiation of Pseudomonas aeruginosa Induces Biogenesis of Outer-Inner Membrane Vesicles With Potential as a Vaccine Against Acute Pneumonia. J Extracell Vesicles, 14(8):e70151(2025) [pubmed]

10.1002/jev2.70151;

submitter keyword: X-ray irradiation, membrane vesicles, proteomics analysis,Pseudomonas aeruginosa

Zhenling Wang
contact affiliation	Sichuan University
contact email	wangzhenling@scu.edu.cn
lab head
Zhenling Wang
contact affiliation	Sichuan University
contact email	wangzhenling@scu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD037062

PRIDE project URI

• PRIDE
  1. PXD037062
     1. Label: PRIDE project
     2. Name: Proteomics analysis of the membrane vesicles in Pseudomonas aeruginosa under X-ray irradiation