PXD037034 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification and functional characterization of proteins interacting with cohesin complex in yeast Saccharomyces cerevisiae. |
Description | To ensure equal separation of DNA, sister chromatids are held together from S phase to metaphase–anaphase transition by a multiprotein complex called cohesin. This makes it possible to establish chromosome biorientation, counteracts the pulling force of mitotic spindle microtubules, preventing premature sister chromatid separation, and ensures precise segregation of sister DNAs into daughter cells In order to better understand how the sister chromatid cohesion process is regulated we looked for new cohesin interacors. We constructed a yeast strain endogenously expressing TAP-tagged Scc1 (Scc1-TAP). Next, we performed a single-step TAP purification using an untagged strain (mock sample) or a strain expressing Scc1-TAP, followed by identification of co-purifying proteins by MS. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_10:21:48.898.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD037034 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | AgataMalinowska |
SpeciesList | scientific name: Olea europaea (Common olive); NCBI TaxID: 4146; scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: 9598; scientific name: Brassica napus (Rape); NCBI TaxID: 3708; scientific name: Halobacterium salinarum; NCBI TaxID: 2242; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036; scientific name: Ictalurus punctatus (Channel catfish); NCBI TaxID: 7998; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; scientific name: Taeniopygia guttata (Zebra finch) (Poephila guttata); NCBI TaxID: 59729; scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hepatitis C virus subtype 1a; NCBI TaxID: 31646; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; scientific name: Merluccius bilinearis (Silver hake); NCBI TaxID: 79698; scientific name: Brassica oleracea var. botrytis (Cauliflower); NCBI TaxID: 3715; scientific name: Pongo pygmaeus (Bornean orangutan); NCBI TaxID: 9600; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Heliocidaris erythrogramma (Sea urchin); NCBI TaxID: 7634; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097; scientific name: Ricinus communis; NCBI TaxID: 3988; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689; scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; scientific name: Struthio camelus (Ostrich); NCBI TaxID: 8801; scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-27 11:27:34 | ID requested | |
⏵ 1 | 2023-10-24 10:21:49 | announced | |
2 | 2023-11-14 09:06:43 | announced | 2023-11-14: Updated project metadata. |
Publication List
Litwin I, Nowicka M, Markowska K, Maciaszczyk-Dziubi, ń, ska E, Tomaszewska P, Wysocki R, Kramarz K, ISW1a modulates cohesin distribution in centromeric and pericentromeric regions. Nucleic Acids Res, 51(17):9101-9121(2023) [pubmed] |
10.6019/PXD037034; |
Keyword List
submitter keyword: Scc1,cohesin complex, cohesion |
Contact List
IreneuszLitwin |
contact affiliation | University of Wrocław, Faculty of Biological Sciences |
contact email | ireneusz.litwin@uwr.edu.pl |
lab head | |
AgataMalinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037034
- Label: PRIDE project
- Name: Identification and functional characterization of proteins interacting with cohesin complex in yeast Saccharomyces cerevisiae.