PXD037031-1

PXD037031 is an original dataset announced via ProteomeXchange.

Title	Urinary endogenous peptides as biomarkers for prostate cancer
Description	Prostate cancer (PCa) is one of the most prevalent cancer types in men worldwide. However, the main diagnostic tests available for PCa have limitations and need biopsy for histopathological confirmation of the disease. The prostate-specific antigen (PSA) is the main biomarker used for PCa early detection, but an elevated serum concentration is not cancer-specific. Therefore, there is a need for discovery of new non-invasive biomarkers that can accurately diagnose PCa. Here, we used trichloroacetic acid-induced protein precipitation and liquid chromatography-mass spectrometry to profile endogenous peptides in urine samples from patients with PCa (n = 33), benign prostatic hyperplasia (n = 25), and healthy individuals (n = 28). Receiver operating characteristic (ROC) curves were performed to evaluate the diagnostic performance of urinary peptides. In addition, proteasix tool was used for in silico prediction of protease cleavage sites. We found five urinary peptides derived from uromodulin significantly altered between the study groups, all of which were less abundant in the PCa group. In addition, urinary peptides outperformed PSA in discriminating between malignant and benign prostate conditions (AUC = 0.847), showing high sensitivity (81.82%) and specificity (88%). Overall, our study allowed the identification of urinary peptides with potential for use as non-invasive biomarkers in PCa diagnosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:36:04.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristine Dutra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:26:45	ID requested
⏵ 1	2024-10-08 11:36:04	announced

Dataset with its publication pending

submitter keyword: uromodulin, urine, endogenous peptides, mass spectrometry, biomarkers,Prostate cancer

Karina Mariante Monteiro
contact affiliation	Laboratory of Structural and Functional Genomics, Biotechnology Center, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
contact email	karina.monteiro@ufrgs.br
lab head
Cristine Dutra
contact affiliation	Universidade Federal do Rio Grande do Sul
contact email	cristinedutra@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037031
     1. Label: PRIDE project
     2. Name: Urinary endogenous peptides as biomarkers for prostate cancer