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PXD037006 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMouse osteoclast secretory lysosomes nLC-ESI-MS/MS
DescriptionTo survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles eluted and processed for 1D in-gel digestion and mass spectrometry.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-25 15:28:38ID requested
12023-01-24 19:11:08announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Lysosome, LRO, Mouse, SPION,Osteoclast, Secretory lysosome
Contact List
contact affiliationThe Bone Biology and Disease Laboratory, The University of Western Australia
contact emailnathan.pavlos@uwa.edu.au
lab head
contact affiliationThe University of Western Australia
contact email21703693@student.uwa.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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