PXD036940-1

PXD036940 is an original dataset announced via ProteomeXchange.

Title	Extracellular matrix educates a tumor macrophage phenotype found in ovarian cancer metastasis
Description	Recent studies have shown the tumor extracellular matrix (ECM) associates with immunosuppression, and that targeting the ECM can improve immune infiltration and immunotherapy response. A question that remains is whether the ECM is directly educating the immune phenotypes seen in cancer. We identified a tumor-associated macrophage (TAM) population correlated with poor prognosis, interruption of the cancer immunity cycle, and tumor ECM composition. To investigate whether ECM was capable of generating the TAM phenotype seen, we developed a decellularized tissue model that retains the native ECM architecture and composition. Macrophages cultured on decellularized ovarian metastasis shared transcriptional profiles with the TAMs found in human tissues. ECM educated macrophages have a tissue remodeling and immunoregulatory phenotype, inducing altered T cell function. We conclude that the tumor ECM is directly educating this macrophage population found in cancer tissues. Therefore, current and emerging cancer therapies that target the tumor ECM may be tailored to improve macrophage phenotype and their downstream regulation of immunity.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_05:43:04.912.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036940
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	VinothiniRajeeve
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-22 00:12:51	ID requested
⏵ 1	2023-04-11 05:43:05	announced
2	2023-11-14 09:01:08	announced	2023-11-14: Updated project metadata.

submitter keyword: cancer, Tumor-associated macrophages, proteomics,Decellularized tissue model, immunity, extracellular matrix, LC-MS/MS

Professor Pedro RCutillas
contact affiliation	Professor of Cell Signalling & Proteomics Centre for Genomics and Computational Biology Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ Tel: +44 (0)20 7882 8266 | Fax: +44 (0)20 7882 3891
contact email	p.cutillas@qmul.ac.uk
lab head
VinothiniRajeeve
contact affiliation	Centre for Haemato Oncology
contact email	v.rajeeve@qmul.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036940
     1. Label: PRIDE project
     2. Name: Extracellular matrix educates a tumor macrophage phenotype found in ovarian cancer metastasis