PXD036908-1

PXD036908 is an original dataset announced via ProteomeXchange.

Title	Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery
Description	Photosynthesis is central to food production and the Earth’s biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii, we identify 115 genes required for photosynthesis, 70 of which were previously uncharacterized. We initiate functional characterization of the novel genes by determining the proteomes of mutant strains lacking these genes. The data allow assignment of 34 of the novel genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Additional analysis uncovers at least seven novel critical regulatory proteins, including five Photosystem I mRNA maturation factors and two master regulators: MTF1, which impacts chloroplast gene expression directly; and PMR1, which impacts expression via nuclear-expressed factors. Our work provides a rich resource identifying novel regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.
HostingRepository	PRIDE
AnnounceDate	2023-08-02
AnnouncementXML	Submission_2023-08-02_06:26:11.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MosheKafri
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 15:09:46	ID requested
⏵ 1	2023-08-02 06:26:11	announced

Dataset with its publication pending

submitter keyword: methionyl-tRNA formyltransferase, protein profiling, RNA-sequencing, retrograde regulation,Photosynthesis, Photosystem I maturation factors, genetics, protein localization.

Martin C.Jonikas
contact affiliation	Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA.
contact email	mjonikas@princeton.edu
lab head
MosheKafri
contact affiliation	Post-doc
contact email	moshekafri@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD036908

PRIDE project URI

• PRIDE
  1. PXD036908
     1. Label: PRIDE project
     2. Name: Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery