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PXD036860-1
PXD036860 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes |
| Description | Transcriptomic analyses revealed hundreds of p53-regulated genes, however these studies used a limited number of cell lines and p53-activating agents. Therefore, we searched for candidate p53-target genes by employing stress factors and cell lines never used in high-throughput search for p53-regulated genes. We performed RNA-Seq on A549 cells exposed either to camptothecin, actinomycin D, nutlin-3a or to a combination of actinomycin D and nutlin-3a (A+N). The latter two substances synergize in activation of selected p53-target genes. Similar analysis was performed on other cell lines (U-2 OS, NCI-H460, A375) exposed to A+N. Mass spectrometry was employed to identify proteins in cell lysates or those secreted to the medium of A549 cells in control conditions or treated with A+N. Expression of selected genes strongly upregulated by A+N or camptothecin was examined by RT-PCR in p53-deficient cells and their controls. We found that p53 participates in upregulation of: ACP5, APOL3, CDH3, CIBAR2, CRABP2, CTHRC1, CTSH, FAM13C, FBXO2, FRMD8, FRZB, GAST, ICOSLG, KANK3, KCNK6, KLRG2, MAFB, MR1, NDRG4, PTAFR, RETSAT, TMEM52, TNFRSF14, TRANK1, TYSND1, WFDC2, WFDC5, WNT4 genes. Twelve of their proteins were detected in the secretome and/or proteome of treated cells. Our data generate new hypotheses concerning functioning of p53 tumor suppressor. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-31 |
| AnnouncementXML | Submission_2026-05-31_02:39:16.490.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Monika Pietrowska |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-09-20 14:44:47 | ID requested | |
| ⏵ 1 | 2026-05-31 02:39:17 | announced |
Publication List
| 10.1016/j.cbi.2024.110946; |
| Ł, asut-Szyszka B, Gdowicz-K, ł, osok A, Ma, ł, achowska B, Krze, ś, niak M, B, ę, dzi, ń, ska A, Gawin M, Pietrowska M, Rusin M, Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes. Chem Biol Interact, 392():110946(2024) [pubmed] |
Keyword List
| submitter keyword: transcriptome, secretome,p53 |
| innate immunity |
| WNT signaling |
Contact List
| Marek Rusin | |
|---|---|
| contact affiliation | Maria Sklodowska-Curie National Research Institute of Oncology |
| contact email | marek.rusin@io.gliwice.pl |
| lab head | |
| Monika Pietrowska | |
| contact affiliation | Maria Sklodowska-Curie National Research Institute of Oncology |
| contact email | monika.pietrowska@io.gliwice.pl |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD036860 |
| PRIDE project URI |
Repository Record List
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