PXD036817-1

PXD036817 is an original dataset announced via ProteomeXchange.

Title	Metabolic reconfiguration through SGLT2 inhibition improves metabolic waste excretion
Description	Dapagliflozin is a member of a new class of medication called gliflozins indicated for the treatment of diabetes and has recently been also approved for the treatment of kidney and heart diseases. Gliflozins lower blood glucose levels by inhibition of the sodium glucose co-transporter 2 (SGLT2), which is predominantly expressed in the S1 segment of renal proximal tubules and responsible for >90% of renal glucose reabsorption. Renal and cardio protection by gliflozins cannot be solely explained by blood glucose lowering. To gain further mechanistic insights, we determined the metabolomic and proteomic signature of dapagliflozin in a murine model of diabetes. Male C57BL/6 non-diabetic wildtype and littermate Ins2 (Akita) diabetic mice, both fed a Western diet for 5 weeks, were given dapagliflozin (10 mg/kg diet) or vehicle for one week (4 groups, N=8/group). Blood and urine glucose levels confirmed the expected treatment response. We analyzed metabolome, proteome and phosphoproteome for a range of tissues (kidney, liver, heart, smooth muscle, white adipose tissue) and body fluids (serum, urine, erythrocytes), and extended the analysis to the gut metaproteome.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_04:43:11.711.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anja Billing
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: mouse gut metagenome; NCBI TaxID: NEWT:410661;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 15:01:54	ID requested
⏵ 1	2026-02-09 04:43:12	announced

10.6019/PXD036817;

Billing AM, Kim YC, Gullaksen S, Schrage B, Raabe J, Hutzfeldt A, Demir F, Kovalenko E, Lass, é M, Dugourd A, Fallegger R, Klampe B, Jaegers J, Li Q, Kravtsova O, Crespo-Masip M, Palermo A, Fenton RA, Hoxha E, Blankenberg S, Kirchhof P, Huber TB, Laugesen E, Zeller T, Chrysopoulou M, Saez-Rodriguez J, Magnussen C, Eschenhagen T, Staruschenko A, Siuzdak G, Poulsen PL, Schwab C, Cuello F, Vallon V, Rinschen MM, Metabolic Communication by SGLT2 Inhibition. Circulation, 149(11):860-884(2024) [pubmed]

10.1161/circulationaha.123.065517;

submitter keyword: White adipose tissue, Kidney, Heart, Skeletal Muscle, LC-MS/MS, Dapagliflozin,Mouse, TMT, Exploris 480, murine, SGLT2 inhibitors, Liver, Gut Microbiome

Markus Rinschen
contact affiliation	Kidney omics and metabolism Institute of Biomedicine Aarhus University Høegh-Guldbergsgade 10 8000 Aarhus C Denmark
contact email	rinschen@aias.au.dk
lab head
Anja Billing
contact affiliation	Aarhus University
contact email	anja.billing@biomed.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD036817

PRIDE project URI

• PRIDE
  1. PXD036817
     1. Label: PRIDE project
     2. Name: Metabolic reconfiguration through SGLT2 inhibition improves metabolic waste excretion