PXD036811-1

PXD036811 is an original dataset announced via ProteomeXchange.

Title	Microbial Peptides Activate Tumor-Infiltrating Lymphocytes in Glioblastoma
Description	Microbial organisms play key roles in numerous physiological processes in the human body and have recently been shown to modify the response to cancer radiotherapy and immune checkpoint inhibitors. Here, we demonstrate that HLA molecules of both glioblastoma tissues as well as tumor cell lines present bacteria-specific peptides. This finding prompted us to examine whether tumor-infiltrating lymphocytes (TILs) recognize intratumoral microbiota. Indeed, bacterial peptides eluted from HLA-DR II molecules are recognized by both TILs, albeit weakly, and peripheral blood-derived memory CD4+ T cells, which, upon stimulation with bacterial peptides, also recognize several tumor antigens. Furthermore, using an unbiased antigen discovery approach for a TIL CD4+ T cell clone (TCC) we show that it recognizes a broad spectrum of peptides from pathogenic bacteria, commensal gut microbiota and also glioblastoma-related tumor antigens. These peptides were strongly stimulatory for bulk TILs and peripheral blood memory cells. Our data hint at how bacterial pathogens and bacterial gut microbiota can be involved in specific immune recognition of tumor antigens.
HostingRepository	PRIDE
AnnounceDate	2023-02-20
AnnouncementXML	Submission_2023-02-20_01:02:18.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RezaNaghavian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 14:44:56	ID requested
⏵ 1	2023-02-20 01:02:19	announced

Dataset with its publication pending

submitter keyword: Human, Glioblastoma, Immunopeptidome, HLA-DR

Professor Dr.RolandMartin
contact affiliation	Neuroimmunology and MS Research Section (NIMS), Neurology Clinic, University of Zurich, University Hospital Zurich, 8091 Zurich, Switzerland
contact email	roland.martin@usz.ch
lab head
RezaNaghavian
contact affiliation	University of Zurich
contact email	rnaghavian@cellerys.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036811

PRIDE project URI

• PRIDE
  1. PXD036811
     1. Label: PRIDE project
     2. Name: Microbial Peptides Activate Tumor-Infiltrating Lymphocytes in Glioblastoma