PXD036810-1

PXD036810 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chemoproteomic target deconvolution of Lipoic acid reveals Histone Deacetylases as the main targets
Description	Here we set out to identify protein targets of Lipoic acid and Lipoamide by chemical proteomics approaches. We generated Lipoamide-based affinity matrices to pulldown target proteins and quantify them via bottom-up proteomics. Use of the same affinity matrix in dose dependent compeition assays allowed us to identify HDACs as potently bound by Lipoic acid and Lipoamide and to derive the affinty of drug target interactions. These findings were confirmed by further competiiton assays with already established HDAC inhibitor based affinity matrices.
HostingRepository	PRIDE
AnnounceDate	2026-06-02
AnnouncementXML	Submission_2026-06-02_03:31:49.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Severin Lechner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 13:49:57	ID requested
⏵ 1	2026-06-02 03:31:50	announced

Publication List

Lechner S, Steimbach RR, Wang L, Deline ML, Chang YC, Fromme T, Klingenspor M, Matthias P, Miller AK, M, é, dard G, Kuster B, Chemoproteomic target deconvolution reveals Histone Deacetylases as targets of (R)-lipoic acid. Nat Commun, 14(1):3548(2023) [pubmed]
10.1038/s41467-023-39151-8;

Lechner S, Steimbach RR, Wang L, Deline ML, Chang YC, Fromme T, Klingenspor M, Matthias P, Miller AK, M, é, dard G, Kuster B, Chemoproteomic target deconvolution reveals Histone Deacetylases as targets of (R)-lipoic acid. Nat Commun, 14(1):3548(2023) [pubmed]

10.1038/s41467-023-39151-8;

Keyword List

submitter keyword: LC-MSMS, Drug target deconvolution,Cancer cell lines

submitter keyword: LC-MSMS, Drug target deconvolution,Cancer cell lines

Contact List

Bernhard Kuster
contact affiliation	Chair of Proteomics and Biolanalytics, TUM School of Life Sciences, Technical University of Munich, Germany
contact email	kuster@tum.de
lab head
Severin Lechner
contact affiliation	Chair of proteomics and Bioanalytics, Technical Univerity Munich
contact email	severin.lechner@tum.de
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD036810
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD036810

PRIDE project URI

Repository Record List

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