PXD036809-1

PXD036809 is an original dataset announced via ProteomeXchange.

Title	The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo
Description	Histones are essential for chromatin packaging and histone supply must be tightly regulated as excess histones are toxic. To drive the rapid cell cycles of the early embryo, however, excess histones are maternally deposited. Therefore, soluble histones must be buffered by histone chaperones but the chaperone necessary to stabilize soluble H3-H4 pools in the Drosophila embryo has yet to be identified. Here, we show that CG8223, the Drosophila ortholog of NASP, is a H3-H4-specific chaperone in the early embryo. NASP specifically binds to H3-H4 in the early embryo. We demonstrate that, while NASP is non-essential in Drosophila, NASP is maternal effect lethal gene. Embryos laid by NASP mutant mothers have a reduce rate of hatching and show defects in early embryogenesis. Critically, soluble H3-H4 pools are degraded in embryos laid by NASP mutant mothers. Our work identifies NASP as the critical H3-H4 histone chaperone in the Drosophila embryo.
HostingRepository	PRIDE
AnnounceDate	2023-02-23
AnnouncementXML	Submission_2023-02-23_07:19:57.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JonathanDavies
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 13:49:39	ID requested
⏵ 1	2023-02-23 07:19:57	announced

Dataset with its publication pending

submitter keyword: histone,NASP, H3-H4, histone chaperone, IP-MS

Jared T.Nordman
contact affiliation	Department of Biological Sciences, Vanderbilt University
contact email	jared.nordman@vanderbilt.edu
lab head
JonathanDavies
contact affiliation	Vanderbilt University
contact email	jonathan.p.davies@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036809

PRIDE project URI

• PRIDE
  1. PXD036809
     1. Label: PRIDE project
     2. Name: The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo