PXD036803-2

PXD036803 is an original dataset announced via ProteomeXchange.

Title	Integrated omic analysis reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock
Description	Circadian rhythmicity in renal function suggests a requirement for circadian adaptations in renal metabolism. We studied circadian changes in renal metabolic pathways using integrated transcriptomic, proteomic and metabolomic analysis performed on control mice and mice deficient in the circadian clock gene Bmal1 in the renal tubule (cKOt mice). Proteins were extracted from whole kidneys of 60 mice. Of these, 30 were conditional knockouts of Arntl (Bmal1) and 30 were of control genotype. They were housed under 12-hours light/12-hours dark cycles and were sacrificed at six different time points: zeitgeber time ZT 0, ZT 4, ZT 8, ZT 12, ZT 16, ZT 20 ( ZT 0 being the time of light on and ZT 12 the time of light off). Five replicates per genotype and time point were analysed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:12.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 13:47:41	ID requested
1	2023-03-15 16:14:05	announced
⏵ 2	2023-11-14 08:44:15	announced	2023-11-14: Updated project metadata.

Bignon Y, Wigger L, Ansermet C, Weger BD, Lagarrigue S, Centeno G, Durussel F, G, ö, tz L, Ibberson M, Pradervand S, Quadroni M, Weger M, Amati F, Gachon F, Firsov D, Multiomics reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock. J Clin Invest, 133(8):(2023) [pubmed]

submitter keyword: mouse kidney

control mice

mice KO for Arntl in the renal tubule

renal circadian proteome

Dmitri Firsov
contact affiliation	Department of Biomedical Sciences, University of Lausanne,Lausanne, Switzerland
contact email	dmitri.firsov@unil.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036803

PRIDE project URI

• PRIDE
  1. PXD036803
     1. Label: PRIDE project
     2. Name: Integrated omic analysis reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock