PXD036802-1

PXD036802 is an original dataset announced via ProteomeXchange.

Title	Shotgun Proteomics Associates Mobile Phone Usage With Molecular-Level Responses Involved In Brain Functioning, Tissue Remodeling, Reproduction And Calcium Absorption Part 2
Description	Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
HostingRepository	PRIDE
AnnounceDate	2026-06-07
AnnouncementXML	Submission_2026-06-07_03:46:10.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NEELANCHAL VAID
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 13:47:15	ID requested
⏵ 1	2026-06-07 03:46:10	announced

Dataset with its publication pending

submitter keyword: Mobile phone, Vitamin-D signaling., Plasminogen activating cascade, Adrenaline and Noradrenalin synthesis, i-TRAQ, Shot gun proteomics, Cholecystokinin receptor signaling, Radio frequency electromagnetic fields, Dopamine mediated receptor signaling, Gonadotropin-releasing hormone pathway

DR. MANISH SONEJA
contact affiliation	DEPARTMENT OF MEDICINE, ALL INDIA INSTITUTE OF MEDICAL SCIENCES, ANSARI NAGAR, NEW DELHI-110029
contact email	manishsoneja@gmail.com
lab head
NEELANCHAL VAID
contact affiliation	ALL INDIA INSTITUTE OF MEDICAL SCIENCES
contact email	vaidneel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD036802

PRIDE project URI

• PRIDE
  1. PXD036802
     1. Label: PRIDE project
     2. Name: Shotgun Proteomics Associates Mobile Phone Usage With Molecular-Level Responses Involved In Brain Functioning, Tissue Remodeling, Reproduction And Calcium Absorption Part 2