PXD036794-1

PXD036794 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SILAC labelled tendon fibroblasts
Description	Monitoring protein abundance using Mass Spectrometry within cells is a widely used technique and commonly used. The abundance however is a static measurement with no real information about the dynamics of those proteins and so clearly missing important information. In order to get a better appreciation of the intricacies of protein homeostasis and turnover, we employed a SILAC labelling approach. Mouse tendon fibroblasts were grown in 13C lysine SILAC for 48 hours and then the incorporation of SILAC heavy label was measured. This provided a large amount of data which could be analysed to provide information about protein turnover.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_02:23:16.992.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036794
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anna Hoyle
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:54:09	ID requested
⏵ 1	2024-05-23 02:23:17	announced
2	2024-10-22 06:41:57	announced	2024-10-22: Updated project metadata.

Publication List

10.1101/2024.05.09.593302;
10.6019/PXD036794;

10.1101/2024.05.09.593302;

10.6019/PXD036794;

Keyword List

submitter keyword: SILAC tendon fibroblasts turnover

submitter keyword: SILAC tendon fibroblasts turnover

Contact List

Joe Swift
contact affiliation	Wellcome Centre for Cell-Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester
contact email	joe.swift@manchester.ac.uk
lab head
Anna Hoyle
contact affiliation	University of Oxford
contact email	anna.hoyle@postgrad.manchester.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036794
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036794

PRIDE project URI

Repository Record List

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