PXD036790-2

PXD036790 is an original dataset announced via ProteomeXchange.

Title	MS-based identification of small proteins in Campylobacter jejuni
Description	The Gram-negative bacterium Campylobacter jejuni is a widespread food-borne pathogen. Knowing the proteins encoded by the bacterial pathogen and how their expression is regulated is essential to understand how it survives, colonizes, and causes diseases. The present study focusses on small proteins (≤ 50-100 amino acids) translated from small open reading frames (sORFs). These poorly annotated components of the genome show emerging roles in bacterial physiology and virulence. Here, the proteome of C. jejuni during exponential growth in a complex medium was analyzed with the proteogenomics workflow presented in Fuchs et al., 2021 with the special focus on small proteins . In combination with different Ribo-Seq approaches new insights into the coding potential of the genome of C. jejuni are provided.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:01.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kucklick
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:52:57	ID requested
1	2024-05-23 10:18:58	announced
⏵ 2	2024-10-22 06:42:02	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ribosome profiling, GELFREE fractionator, sORF, small proteins, GeLC-MS/MS, Campylobacter jejuni, translatome

Susanne Engelmann
contact affiliation	Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
contact email	susanne.engelmann@helmholtz-hzi.de
lab head
Martin Kucklick
contact affiliation	TU Braunschweig Institut für Mikrobiologie Spielmannstr. 7 Germany
contact email	m.kucklick@tu-braunschweig.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036790

PRIDE project URI

• PRIDE
  1. PXD036790
     1. Label: PRIDE project
     2. Name: MS-based identification of small proteins in Campylobacter jejuni