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PXD036746-2

PXD036746 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunction of the Rh5 complex in invasion of human erythrocytes
DescriptionThe most severe form of malaria is caused by Plasmodium falciparum. These parasites invade human erythrocytes and an essential step in this process involves the ligand PfRh5, which forms a complex with CyRPA and PfRipr (RCR complex) and binds basigin on the host cell. We identified a heteromeric disulphide-linked complex consisting of PfPTRAMP and PfCSS and have shown it binds RCR to form a pentameric complex PCRCR. Using P. falciparum lines with conditional knockouts and invasion inhibitory nanobodies to both PfPTRAMP and PfCSS we utilised lattice light-sheet microscopy and show they are essential for merozoite invasion. The PCRCR complex functions to anchor the contact between merozoite and erythrocyte membranes brought together by strong parasite deformations. We solved the structure of nanobody/PfCSS complexes to identify an inhibitory epitope. Our results define the function of the PCRCR complex and identify invasion neutralising epitopes providing a roadmap for structure-guided development of these proteins for a blood stage malaria vaccine.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:59:11.679.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Dagley
SpeciesList scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; timsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-14 14:27:08ID requested
12022-12-14 15:33:52announced
22023-11-14 08:59:12announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PfRipr, basigin, PTRAMP, human erythrocytes, invasion complex, PfRh5,malaria, CSS, P. falciparum
Contact List
Alan Cowman
contact affiliationDeputy Director – Science Strategy The Walter and Eliza Hall institute of Medical Research Professor, Department of Medical Biology Faculty of Medicine, Dentistry and Health The University of Melbourne Australia
contact emailcowman@wehi.edu.au
lab head
Laura Dagley
contact affiliationWEHI
contact emaildagley.l@wehi.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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