PXD036741 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Syntaxin5’s flexibility in SNARE pairing supports Golgi functions |
Description | The intracellular transport system is an evolutionally conserved, essential, and highly regulated network of organelles and small transport carriers that traffic protein and lipid cargoes within the cell. The Conserved Oligomeric Golgi (COG) complex is the major Golgi Multisubunit Tethering Complex. Its key function is orchestration of SNARE mediated fusion of cargo carriers at the Golgi. We hypothesized the depletion of the major Golgi SNAREs GS28 (GOSR1) and GS15 (BET1L) due to COG malfunction is the major contributor to COG-related glycosylation defects. To test this, we created single, double and triple knockouts (KO) of Golgi SNAREs in HEK293T cells and analyzed the resulting mutants using a comprehensive set of biochemical, mass-spectrometry (MS) and microscopy approaches. Deletion of GS28 significantly affected GS15, but not the other two partners, STX5 and YKT6. Surprisingly, our analysis revealed that COG dysfunction is more deleterious for Golgi function than disrupting the canonical Golgi SNARE complex indicating the existence of an adaption mechanism. Indeed, quantitative mass-spectrometry analysis of STX5-interacting proteins revealed unexpected flexibility in Golgi SNARE pairing in mammalian cells. We uncovered two novel non-canonical Golgi SNARE complexes – STX5/SNAP29/VAMP7 and STX5/VTI1B/GS15/YKT6 which were increased in GS28 KO cells. Upon GS28 deletion, SNAP29 remarkably localized to the Golgi. Merely mislocalizing STX5 from Golgi abolished the SNARE substitution causing dramatic glycosylation defects. Our data points to the remarkable plasticity in the intra-Golgi membrane fusion machinery and places STX5 as the only essential Golgi Qa-SNARE. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:52:29.245.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zinia DSouza |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-14 14:18:21 | ID requested | |
1 | 2023-06-25 20:29:20 | announced | |
⏵ 2 | 2023-11-14 06:52:30 | announced | 2023-11-14: Updated project metadata. |
Publication List
D'Souza Z, Pokrovskaya I, Lupashin VV, Syntaxin-5's flexibility in SNARE pairing supports Golgi functions. Traffic, 24(8):355-379(2023) [pubmed] |
Keyword List
submitter keyword: membrane trafficking, Golgi, BET1L, VTI1B, SNAP29, GOR1, GOSR2, COG,SNAREs, STX5, YKT6 |
Contact List
Vladimir V Lupashin |
contact affiliation | Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, AR 72205, USA. |
contact email | vvlupashin@uams.edu |
lab head | |
Zinia DSouza |
contact affiliation | University of Arkansas for Medical Sciences |
contact email | ZCDsouza@uams.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036741
- Label: PRIDE project
- Name: Syntaxin5’s flexibility in SNARE pairing supports Golgi functions