PXD036676-1

PXD036676 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus prophage-encoded protein causes abortive infection and provides population immunity against kayviruses
Description	The aim of the study was to investigate the resistance mechanism of Staphylococcus aureus towards lytic phages of the genus Kayvirus and the role of the membrane-anchored protein (primary accession Q2FYE0) designated PdpSau encoded by Staphylococcus aureus prophages. PdpSau does not prevent the infecting kayvirus from adsorbing onto the host cell and delivering its genome into the cell, but phage DNA replication is halted. Changes in the cell membrane polarity and permeability were observed 10 min after the infection leading to prophage-activated cell death. The LC-MS/MS analysis, as one of the methods, was used for protein detection and to find out whether this protein is predominantly presented in membranes. These findings are relevant for the advancement of phage therapy.
HostingRepository	PRIDE
AnnounceDate	2023-02-03
AnnouncementXML	Submission_2023-02-03_07:24:56.238.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PetrGintar
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 06:45:39	ID requested
⏵ 1	2023-02-03 07:24:56	announced
2	2023-11-14 08:59:02	announced	2023-11-14: Updated project metadata.

submitter keyword: Kayvirus, PdpSau, Phage resistance, Orbitrap,Staphylococcus aureus, LC-MS/MS

ZbynekZdrahal
contact affiliation	CEITEC Masaryk university
contact email	zdrahal@sci.muni.cz
lab head
PetrGintar
contact affiliation	CEITEC
contact email	460366@muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036676
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus prophage-encoded protein causes abortive infection and provides population immunity against kayviruses