PXD036649-1

PXD036649 is an original dataset announced via ProteomeXchange.

Title	Analysis of bilirubin-induced bovine serum albumin stability using protein denaturation and quantitative cross-linking mass spectrometry
Description	We report the combination of protein-denaturation stability principles with quantitative cross-linking mass spectrometry using isobaric quantitative protein interaction reporter technologies. This method enables the evaluation of ligand-induced protein engagement through analysis of cross-link relative ratios across chemical denaturation. We found ligand-stabilized cross-linked lysine pairs in bovine serum albumin (BSA) and ligand bilirubin map to known binding sites Sudlow Site I and subdomain IB.
HostingRepository	PRIDE
AnnounceDate	2024-07-03
AnnouncementXML	Submission_2024-07-03_02:06:15.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Mathay
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 05:38:46	ID requested
⏵ 1	2024-07-03 02:06:16	announced
2	2024-10-22 06:48:35	announced	2024-10-22: Updated project metadata.

Mathay M, Keller A, Bruce JE, Studying Protein-Ligand Interactions by Protein Denaturation and Quantitative Cross-Linking Mass Spectrometry. Anal Chem, 95(25):9432-9436(2023) [pubmed]

10.1021/acs.analchem.2c04501;

submitter keyword: Quantitative cross-linking, protein stability, Mass Spectrometry

James E Bruce
contact affiliation	Department of Genome Sciences, University of Washington
contact email	jimbruce@uw.edu
lab head
Martin Mathay
contact affiliation	University of Washington
contact email	mmathay1@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD036649

PRIDE project URI

• PRIDE
  1. PXD036649
     1. Label: PRIDE project
     2. Name: Analysis of bilirubin-induced bovine serum albumin stability using protein denaturation and quantitative cross-linking mass spectrometry