PXD036648 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell-based characterization of caspase-3 cleavage sites by LATE and HYATNE |
Description | Proteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides. We applied LATE in combination with other N-terminomic method to study caspase-3 mediated proteolysis both in vitro and during apoptosis in cells. This enable us to identify many unreported caspase-3 cleavages, including some that cannot be identified by other methods. Furthermore, we find direct evidence that some of the caspase-3 generated neo-N-termini can be further modified by Nt-acetylation. This provides a global picture of the caspase-3 degradome and uncovers previously unrecognised functional crosstalk between post-translational Nt-acetylation and caspase proteolysis pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:50:53.914.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oded Kleifeld |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2x(13)C,4x(2)H labeled dimethylated residue; 2x(13)C,4x(2)H labeled dimethylated L-lysine; acetylated residue; dimethylated residue; iodoacetamide derivatized residue; N6,N6-dimethyl-L-lysine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-12 05:38:29 | ID requested | |
1 | 2024-10-07 13:47:37 | announced | |
⏵ 2 | 2024-10-22 05:50:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.mcpro.2023.100584; |
Hanna R, Rozenberg A, Saied L, Ben-Yosef D, Lavy T, Kleifeld O, In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation. Mol Cell Proteomics, 22(7):100584(2023) [pubmed] |
Keyword List
submitter keyword: LATE, HYTANE, ABT199,LysN, Peptidyl-Lys metalloendopeptidase N-terminomics, Caspase-3 |
Contact List
Oded Kleifeld |
contact affiliation | Faculty of Biology Technion - Israel Institute of Technology Haifa, Israel |
contact email | okleifeld@technion.ac.il |
lab head | |
Oded Kleifeld |
contact affiliation | Technion |
contact email | okleifeld@technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036648
- Label: PRIDE project
- Name: Cell-based characterization of caspase-3 cleavage sites by LATE and HYATNE