PXD036646 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Epidermal Stratification Requires Retromer-Mediated Desmoglein-1 Recycling |
Description | Sorting transmembrane cargo is essential for tissue development and homeostasis. However, mechanisms of intracellular trafficking in stratified epidermis are poorly understood. Here we identify an interaction between the retromer endosomal trafficking component, VPS35, and the desmosomal cadherin, Desmoglein-1 (Dsg1). Dsg1 is specifically expressed in stratified epidermis and when properly localized on the plasma membrane of basal keratinocytes, promotes stratification. We show that the retromer drives Dsg1 recycling from the endo-lysosomal system to the plasma membrane to support stratification. The retromer-enhancing chaperone, R55, promotes the membrane localization of Dsg1 and a trafficking-deficient mutant associated with a severe inflammatory skin disorder, enhancing its ability to promote stratification. In the absence of Dsg1, retromer association with and expression of the glucose transporter GLUT1 increases, exposing a potential link between Dsg1 deficiency and epidermal metabolism. Our work provides the first evidence for retromer function in epidermal regeneration, identifying it as a potential therapeutic target. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-24 |
AnnouncementXML | Submission_2023-07-24_13:05:36.550.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DonaldWolfgeher |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-12 05:37:53 | ID requested | |
⏵ 1 | 2023-07-24 13:05:37 | announced | |
2 | 2023-11-14 09:04:14 | announced | 2023-11-14: Updated project metadata. |
Publication List
Hegazy M, Koetsier JL, Huffine AL, Broussard JA, Godsel BM, Cohen-Barak E, Sprecher E, Wolfgeher DJ, Kron SJ, Godsel LM, Green KJ, Epidermal stratification requires retromer-mediated desmoglein-1 recycling. Dev Cell, 57(24):2683-2698.e8(2022) [pubmed] |
Keyword List
submitter keyword: desmosome |
keratinocyte |
epidermal differentiation |
skin disease |
endosomal trafficking |
retromer chaperone |
VPS35 |
SAM syndrome |
cadherin |
Dsg1 |
GLUT1 |
BioID |
Contact List
Dr. Kathleen J.Green |
contact affiliation | 1) Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA 2) Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA 3) Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA |
contact email | kgreen@northwestern.edu |
lab head | |
DonaldWolfgeher |
contact affiliation | University of Chicago |
contact email | donw@uchicago.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD036646 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036646
- Label: PRIDE project
- Name: Epidermal Stratification Requires Retromer-Mediated Desmoglein-1 Recycling