PXD036610-2

PXD036610 is an original dataset announced via ProteomeXchange.

Title	Protein interactors of MEG-1 in C. elegans embryos
Description	In animals with germ plasm, specification of the germline involves “germ granules”, cytoplasmic condensates that enrich maternal transcripts in the germline founder cells. In C. elegans embryos, P granules enrich maternal transcripts, but surprisingly P granules are not essential for germ cell fate specification. Here we have described a second condensate in the C. elegans germ plasm. Like canonical P-bodies found in somatic cells, “germline P-bodies” contain regulators of mRNA decapping and deadenylation and, in addition, the intrinsically-disordered proteins MEG-1 and MEG-2 and the TIS11-family RNA-binding protein POS-1. Embryos lacking meg-1 and meg-2 do not stabilize P-body components, miss-regulate POS-1 targets, miss-specify the germline founder cell, and do not develop a germline. Our findings suggest that specification of the germ line involves at least two distinct condensates that independently enrich and regulate maternal mRNAs in the germline founder cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:20.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Madeline Cassani
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:38:41	ID requested
1	2022-10-04 16:45:27	announced
⏵ 2	2023-11-14 08:59:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: C. elegans, germ granules, MEG-1, p-bodies

Geraldine Seydoux
contact affiliation	Johns Hopkins University School of Medicine
contact email	gseydoux@jhmi.edu
lab head
Madeline Cassani
contact affiliation	Johns Hopkins University
contact email	mcassan1@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036610
     1. Label: PRIDE project
     2. Name: Protein interactors of MEG-1 in C. elegans embryos