<<< Full experiment listing


PXD036610 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein interactors of MEG-1 in C. elegans embryos
DescriptionIn animals with germ plasm, specification of the germline involves “germ granules”, cytoplasmic condensates that enrich maternal transcripts in the germline founder cells. In C. elegans embryos, P granules enrich maternal transcripts, but surprisingly P granules are not essential for germ cell fate specification. Here we have described a second condensate in the C. elegans germ plasm. Like canonical P-bodies found in somatic cells, “germline P-bodies” contain regulators of mRNA decapping and deadenylation and, in addition, the intrinsically-disordered proteins MEG-1 and MEG-2 and the TIS11-family RNA-binding protein POS-1. Embryos lacking meg-1 and meg-2 do not stabilize P-body components, miss-regulate POS-1 targets, miss-specify the germline founder cell, and do not develop a germline. Our findings suggest that specification of the germ line involves at least two distinct condensates that independently enrich and regulate maternal mRNAs in the germline founder cells.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMadeline Cassani
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-12 03:38:41ID requested
12022-10-04 16:45:27announced
22023-11-14 08:59:21announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: C. elegans, germ granules, MEG-1, p-bodies
Contact List
Geraldine Seydoux
contact affiliationJohns Hopkins University School of Medicine
contact emailgseydoux@jhmi.edu
lab head
Madeline Cassani
contact affiliationJohns Hopkins University
contact emailmcassan1@jh.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036610
PRIDE project URI
Repository Record List
[ + ]