PXD036512-1

PXD036512 is an original dataset announced via ProteomeXchange.

Title	Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signaling
Description	In the mammalian DNA damage response, ADP-ribosylation signaling is of crucial importance to mark sites of DNA damage as well as recruit and regulate repairs factors. Specifically, the PARP1:HPF1 complex recognizes damaged DNA and catalyzes the formation of serine-linked ADP-ribosylation marks (mono-Ser-ADPr), which are extended into ADP-ribose polymers (poly-Ser-ADPr) by PARP1 alone. Poly-Ser-ADPr is reversed by PARG, while the terminal mono-Ser-ADPr is removed by ARH3. Despite its significance and apparent evolutionary conservation, little is known about ADP-ribosylation signaling in non-mammalian Animalia. The presence of HPF1, but absence of ARH3, in some insect genomes, including Drosophila species, raises questions regarding the existence and reversal of serine-ADP-ribosylation in these species. Here we show by quantitative proteomics that Ser-ADPr is the major form of ADP-ribosylation in the DNA damage response of Drosophila melanogaster and is dependent on the Parp1:Hpf1 complex. Moreover, our structural and biochemical data reveal a new mechanism of mono-Ser-ADPr removal by Drosophila Parg.
HostingRepository	PRIDE
AnnounceDate	2023-05-26
AnnouncementXML	Submission_2023-05-26_12:54:47.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SaraLarsen
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:09:41	ID requested
⏵ 1	2023-05-26 12:54:48	announced
2	2024-10-03 03:37:43	announced	2024-10-03: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, ADPr, EThcD, PARP, Drosophila, PARG, Af1521,ADP-ribosylation

Michael LundNielsen
contact affiliation	Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
SaraLarsen
contact affiliation	NNF Center for Protein Research
contact email	sara.larsen@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036512

PRIDE project URI

• PRIDE
  1. PXD036512
     1. Label: PRIDE project
     2. Name: Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signaling