PXD036507-1

PXD036507 is an original dataset announced via ProteomeXchange.

Title	Glycosylation Patterns on Collagen α1(I) and Collagen α2(I) resemble a lock and key arrangement
Description	Col1 from the skins of 4 animals (cow, deer, goat and sheep) were purified, then used mass spectrometry and proteomic techniques to identify lysines that were oxidised, galactosylated, glucosylgalactosylated, or glycated in its ma-ture sequence. We found 18 out of the 38 lysines in collagen type 1α1, (Col1A1) and 7 of the 30 lysines in collagen type 1α2 (Col1A2) were glycosylated. Six of these modifications had not been reported before, and included a lysine involved in cross linking collagen molecules. Mapping the positions of these modifications on the ColA1 and Col1A2 sequences showed they were complimentary and resembled a lock and key arrangement.
HostingRepository	PRIDE
AnnounceDate	2023-05-24
AnnouncementXML	Submission_2023-05-23_17:12:30.685.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TrevorLoo
SpeciesList	scientific name: Capra hircus x Ovis aries; NCBI TaxID: 2666255; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	carbamoylated residue; hexose glycated L-lysine; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 08:55:13	ID requested
⏵ 1	2023-05-23 17:12:31	announced
2	2023-11-14 08:57:37	announced	2023-11-14: Updated project metadata.

submitter keyword: hydroxylation, in-gel digestion,glycosylation, lysine, collagen, type 1

GillNorris
contact affiliation	Massey University
contact email	G.Norris@massey.ac.nz
lab head
TrevorLoo
contact affiliation	Massey University
contact email	t.s.loo@massey.ac.nz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036507
     1. Label: PRIDE project
     2. Name: Glycosylation Patterns on Collagen α1(I) and Collagen α2(I) resemble a lock and key arrangement