PXD036504-3

PXD036504 is an original dataset announced via ProteomeXchange.

Title	The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile
Description	Polyamines (PAs) and proteins have been demonstrated to be fundamental for in vitro shoot development of Cedrela fissilis. We evaluated the influence of 6-benzyladenine (BA) and putrescine (Put) on the growth of shoots, PA metabolism and proteomic profiles of C. fissilis. The longest shoots were obtained under 2.5 μM BA + 2.5 mM Put treatment. The inhibition of Put synthesis by D-arginine (D-arg) decreased the shoot length and reduced the endogenous contents of free Put and the activities of the Put biosynthesis enzymes arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), resulting in reduced shoot growth. The ODC activity was higher than that of ADC, which is the main enzyme in the synthesis of Put in C. fissilis. Inhibition of Put synthesis affected the proteomic profile, reducing the accumulation of the ubiquitin receptor RAD23c, peroxidase 15, ADP-ribosylation factor 1, ADP-ribosylation factor-like protein 8a, profilin-4, profilin-2, glucan endo-1,3-beta-glucosidase, and expansin-like B1 and increasing the accumulation of V-type proton ATPase catalytic subunit A and methionine gamma-lyase, highlighting the relevance of these proteins in increasing shoot length. The transport protein SEC13 homolog B and the basic isoform glucan endo-1,3-beta-glucosidase, which are unique to shoots treated with BA+Put, are related to the promotion of shoot growth. Our results show that the modulation of endogenous PAs and proteomic profiles is necessary to regulate in vitro morphogenesis in C. fissilis. Moreover, the ODC enzyme is highly involved in the synthesis of Put during in vitro shoot development and is described for the first time in this species.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:42.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudete Santa-Catarina
SpeciesList	scientific name: Cedrela fissilis; NCBI TaxID: 281395;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 08:52:30	ID requested
1	2023-09-06 09:58:51	announced
2	2023-11-14 07:56:33	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:02:42	announced	2024-10-22: Updated project metadata.

10.1007/S11240-022-02414-1;

submitter keyword: Polyamine inhibitor, Morphogenic competence, Enzymatic activities,Putrescine, Proteomics

Claudete Santa Catarina
contact affiliation	Universidade Estadual do Norte Fluminense Darcy Ribeiro Laboratório de Biologia Celular ou Tecidual Av. Alberto Lamego 2000 - Campos dos Goytacazes-RJ 28013-602 - Brazil (lab head)
contact email	claudete@uenf.br
lab head
Claudete Santa-Catarina
contact affiliation	Universidade Estadual do Norte Fluminense Darcy Ribeiro
contact email	claudete@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036504
     1. Label: PRIDE project
     2. Name: The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile