PXD036480-1

PXD036480 is an original dataset announced via ProteomeXchange.

Title	Quantitative LC-MSMS of new hepatoma HepaRG-derived cells obtained using compression forces
Description	HepaRG progenitor cells are liver carcinoma cells that exhibit transdifferentiation properties, however, the underlying mechanisms remain poorly known. To evaluate whether a compression stress that mimics the tumoral microenvironment can drive the transdifferentiation of HepaRG cells, we analysed their proteome after modulating the density at which they are grown (compression at high density) and their hormonal environment. We determined a molecular signature of the new HepaRG-derived cell lines, reflecting their differentiation status and stress- and chromatin-related responses. In addition to be a promising way of creating a variety of new cell lines for the study of, e.g., tumoral progression, physical constraint paves the way for the development of new solutions to improve the prognosis of cancer.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_00:01:52.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabrice BERTILE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 06:45:44	ID requested
⏵ 1	2024-05-23 00:01:53	announced
2	2024-10-22 06:42:07	announced	2024-10-22: Updated project metadata.

Brun C, Allain C, Ferron PJ, Younoussa H, Colicchio B, Jeandidier E, M'Kacher R, Guguen-Guillouzo C, Bertile F, Extended lifespan and improved genome stability in HepaRG-derived cell lines through reprogramming by high-density stress. Proc Natl Acad Sci U S A, 120(36):e2219298120(2023) [pubmed]

10.1073/pnas.2219298120;

submitter keyword: LC-MSMS, transdifferentiation,Mechanotransduction, liver cell lines, proteome

Sarah Cianférani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique, Analytical Science department, IPHC, CNRS, Strasbourg University, UMR7178, Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Fabrice BERTILE
contact affiliation	CNRS
contact email	fbertile@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036480

PRIDE project URI

• PRIDE
  1. PXD036480
     1. Label: PRIDE project
     2. Name: Quantitative LC-MSMS of new hepatoma HepaRG-derived cells obtained using compression forces