PXD036450-2

PXD036450 is an original dataset announced via ProteomeXchange.

Title	Diadenosine tetraphosphate as a cosubstrate for AMPylation mediated by FICD
Description	Diadenosine polyphosphates (ApnAs) are noncanonical nucleotides that are ubiquitous across all forms of life. Due to their strong accumulation upon cellular stress, they are considered alarmones to signal homeostatic imbalance. Nonetheless, their cellular role is poorly understood. In this work, we assessed ApnAs for their usage as cosubstrate for protein AMPylation, a post-translational modification. In humans, AMPylation mediated by the AMPylator FICD with ATP as cosubstrate is a response to ER stress. Here, we demonstrate that Ap4A is proficiently consumed for AMPylation by FICD. By chemical proteomics using a new chemical probe, we identified new potential AMPylation targets. Interestingly, we found that AMPylation targets of FICD may differ depending on the nucleotide cosubstrate. These results may suggest that signaling at elevated Ap4A levels during cellular stress differs from when Ap4A is present at low concentrations allowing response to extracellular cues.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:05.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Saumer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; adenylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 04:56:06	ID requested
1	2023-01-27 00:25:48	announced
⏵ 2	2023-11-14 08:58:05	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Diadenosine tetraphosphate, AMPylation, FICD, Post-translational modification, Proteomics

Andreas Marx
contact affiliation	Organic Chemistry / Cellular Chemistry, Dept. of Chemistry, University of Konstanz (Germany)
contact email	andreas.marx@uni-konstanz.de
lab head
Philip Saumer
contact affiliation	University of Konstanz
contact email	philip.saumer@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036450
     1. Label: PRIDE project
     2. Name: Diadenosine tetraphosphate as a cosubstrate for AMPylation mediated by FICD