PXD036448-1

PXD036448 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The Role of LVV-H7 in Alcohol-Induced Reward Mechanisms
Description	Hemorphins are short peptides generated during cleavage of the hemoglobin chains but alpha chain, with various functions in different systems and tissues in both physiology and pathophysiology. The main purpose of this study was to establish the role of LVV-H7 in the mechanism of alcohol dependence. LVV-hemorphin 7 (LVV-H7) is released at higher concentrations in the presence of alcohol in vitro. Therefore, we predicted that alcohol intake can change the level of LVV-H7. We have accomplished behavioral tests in animal studies, along with proteomic analysis of the CNS tissues of alcohol addicted rats to verify the correlation of LVV-H7 level and ethanol dependence. We have also confirmed the BBB permeability of synthesized LVV-H7, as well as an inhibitor of its releasing enzyme- pepstatin using fluorescent microscopy. We have found a dose-dependent correlation between LVV-H7 and the time spent by rats in the drug-associated compartment using CPP test. Utilizing mass spectrometry-based methods we identified protein binding targets of LVV-H7 in the CNS tissue of addicted rats, along with the LVV-H7 degrading enzymes. The interpretation of the obtained results reveals the substantial role of LVV-H7 in the mechanism of alcohol dependence.
HostingRepository	PRIDE
AnnounceDate	2025-09-05
AnnouncementXML	Submission_2025-09-05_06:10:36.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Przemyslaw Mielczarek
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 04:55:24	ID requested
⏵ 1	2025-09-05 06:10:37	announced

Publication List

10.1111/ADB.70086;

10.1111/ADB.70086;

Keyword List

submitter keyword: LVV-H7
hemorphins
alcohol
addiction
binding targets
MALDI
progteomics

submitter keyword: LVV-H7

hemorphins

alcohol

addiction

binding targets

MALDI

progteomics

Contact List

Przemyslaw Mielczarek
contact affiliation	1) AGH University of Krakow, Krakow, Poland 2) Maj Institute of Pharmacology, Polish Academy od Sciences, Krakow, Poland
contact email	przemyslaw.mielczarek@agh.edu.pl
lab head
Przemyslaw Mielczarek
contact affiliation	Department of Analytical Chemistry and Biochemistry, AGH University of Science and Technology, Krakow Poland Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland
contact email	przemyslaw.mielczarek@agh.edu.pl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD036448
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD036448

PRIDE project URI

Repository Record List

[ + ]