PXD036446-1

PXD036446 is an original dataset announced via ProteomeXchange.

Title	HDX-MS of AcrIF9 and AcrIF2 binding to CRISPR IF Csy complex
Description	CRISPR RNA-guided detection and degradation of foreign DNA is a dynamic process. Viruses can interfere with this cellular defense by expressing small proteins called anti-CRISPRs. While structural models of anti-CRISPRs bound to their target complex provide static snapshots that inform mechanism, the dynamics and thermodynamics of these interactions are often overlooked. Here we use hydrogen deuterium exchange-mass spectrometry (HDX-MS) and differential scanning fluorimetry (DSF) experiments to determine how anti-CRISPR binding impacts the conformational landscape of the type IF CRISPR RNA guided surveillance complex (Csy) upon binding of two different anti-CRISPR proteins (AcrIF9 and AcrIF2). The results demonstrate that AcrIF2 binding relies on enthalpic stabilization, whereas AcrIF9 uses an entropy driven reaction to bind the CRISPR RNA-guided surveillance complex. Collectively, this work reveals the thermodynamic basis and mechanistic versatility of anti-CRISPR-mediated immune suppression. More broadly, this work presents a striking example of how allosteric effectors are employed to regulate nucleoprotein complexes.
HostingRepository	PRIDE
AnnounceDate	2022-11-01
AnnouncementXML	Submission_2022-11-01_06:58:00.240.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AngelaPatterson
SpeciesList	scientific name: Pseudomonas aeruginosa N002; NCBI TaxID: 1194669;
ModificationList	No PTMs are included in the dataset
Instrument	Agilent instrument model; Bruker Daltonics micrOTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-01 02:10:37	ID requested
⏵ 1	2022-11-01 06:58:00	announced
2	2023-11-14 08:59:06	announced	2023-11-14: Updated project metadata.

submitter keyword: Csy,HDX, Acr

BrianBothner
contact affiliation	Department of Chemistry and Biochemistry Montana State University
contact email	bbothner@montana.edu
lab head
AngelaPatterson
contact affiliation	Indiana University (Bloomington, IN)
contact email	ap4@iusb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD036446

PRIDE project URI

• PRIDE
  1. PXD036446
     1. Label: PRIDE project
     2. Name: HDX-MS of AcrIF9 and AcrIF2 binding to CRISPR IF Csy complex