PXD036442-1
PXD036442 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Stability of the neuronal fate determinant MEIS2 is controlled by calpain2 |
Description | Calpains are non-lysosomal, Ca2+-dependent cysteine proteases, which are associated with various cellular functions but have so far been mainly studied in the context of disease. Their contribution to homeostasis in the healthy organism is still not well understood and their substrates have remained enigmatic in most cases. In the present study, we describe a previously unrecognized role for the calpain protease calpain2 in the regulation of neuronal differentiation of adult neural stem- and progenitor cells through cleavage and elimintation of the neuronal fate determinant MEIS2. Mass spectrometry analysis was performed on immunoprecipitated MEIS2 protein to identify phosphory¬lated residues in MEIS2 and on immunoprecipitated MEIS2 incubated with native porcine calpain2 to map calpain2-induced cleavage sites in the protein. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-08 |
AnnouncementXML | Submission_2024-02-08_06:21:42.679.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036442 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ilka Wittig |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-09-01 02:07:26 | ID requested | |
⏵ 1 | 2024-02-08 06:21:43 | announced |
Publication List
10.1242/jcs.261482; |
10.6019/PXD036442; |
M, ü, ller T, Reichlmeir M, Hau AC, Wittig I, Schulte D, The neuronal transcription factor MEIS2 is a calpain-2 protease target. J Cell Sci, 137(4):(2024) [pubmed] |
Keyword List
submitter keyword: stem cell, protease cleavage sites, MEIS2,Calpain, subventricular zone, phosphoproteomics, adult neurogenesis |
Contact List
Dorothea Schulte | |
---|---|
contact affiliation | Institute of Neurology (Edinger Institute), University Hospital Frankfurt, Goethe University, Frankfurt, Germany |
contact email | Dorothea.Schulte@kgu.de |
lab head | |
Ilka Wittig | |
contact affiliation | Functional Proteomics, Goethe University, Frankfurt am Main , Germany |
contact email | wittig@med.uni-frankfurt.de |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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