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PXD036442 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStability of the neuronal fate determinant MEIS2 is controlled by calpain2
DescriptionCalpains are non-lysosomal, Ca2+-dependent cysteine proteases, which are associated with various cellular functions but have so far been mainly studied in the context of disease. Their contribution to homeostasis in the healthy organism is still not well understood and their substrates have remained enigmatic in most cases. In the present study, we describe a previously unrecognized role for the calpain protease calpain2 in the regulation of neuronal differentiation of adult neural stem- and progenitor cells through cleavage and elimintation of the neuronal fate determinant MEIS2. Mass spectrometry analysis was performed on immunoprecipitated MEIS2 protein to identify phosphory┬Člated residues in MEIS2 and on immunoprecipitated MEIS2 incubated with native porcine calpain2 to map calpain2-induced cleavage sites in the protein.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterIlka Wittig
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-01 02:07:26ID requested
12024-02-08 06:21:43announced
Publication List
M, ├╝, ller T, Reichlmeir M, Hau AC, Wittig I, Schulte D, The neuronal transcription factor MEIS2 is a calpain-2 protease target. J Cell Sci, 137(4):(2024) [pubmed]
Keyword List
submitter keyword: stem cell, protease cleavage sites, MEIS2,Calpain, subventricular zone, phosphoproteomics, adult neurogenesis
Contact List
Dorothea Schulte
contact affiliationInstitute of Neurology (Edinger Institute), University Hospital Frankfurt, Goethe University, Frankfurt, Germany
contact emailDorothea.Schulte@kgu.de
lab head
Ilka Wittig
contact affiliationFunctional Proteomics, Goethe University, Frankfurt am Main , Germany
contact emailwittig@med.uni-frankfurt.de
dataset submitter
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Dataset FTP location
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