PXD036442

PXD036442 is an original dataset announced via ProteomeXchange.

Title	Stability of the neuronal fate determinant MEIS2 is controlled by calpain2
Description	Calpains are non-lysosomal, Ca2+-dependent cysteine proteases, which are associated with various cellular functions but have so far been mainly studied in the context of disease. Their contribution to homeostasis in the healthy organism is still not well understood and their substrates have remained enigmatic in most cases. In the present study, we describe a previously unrecognized role for the calpain protease calpain2 in the regulation of neuronal differentiation of adult neural stem- and progenitor cells through cleavage and elimintation of the neuronal fate determinant MEIS2. Mass spectrometry analysis was performed on immunoprecipitated MEIS2 protein to identify phosphory¬lated residues in MEIS2 and on immunoprecipitated MEIS2 incubated with native porcine calpain2 to map calpain2-induced cleavage sites in the protein.
HostingRepository	PRIDE
AnnounceDate	2024-02-08
AnnouncementXML	Submission_2024-02-08_06:21:42.679.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036442
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-01 02:07:26	ID requested
⏵ 1	2024-02-08 06:21:43	announced

10.1242/jcs.261482;

10.6019/PXD036442;

M, ü, ller T, Reichlmeir M, Hau AC, Wittig I, Schulte D, The neuronal transcription factor MEIS2 is a calpain-2 protease target. J Cell Sci, 137(4):(2024) [pubmed]

submitter keyword: stem cell, protease cleavage sites, MEIS2,Calpain, subventricular zone, phosphoproteomics, adult neurogenesis

Dorothea Schulte
contact affiliation	Institute of Neurology (Edinger Institute), University Hospital Frankfurt, Goethe University, Frankfurt, Germany
contact email	Dorothea.Schulte@kgu.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD036442

PRIDE project URI

• PRIDE
  1. PXD036442
     1. Label: PRIDE project
     2. Name: Stability of the neuronal fate determinant MEIS2 is controlled by calpain2