PXD036359-2

PXD036359 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of glioblastoma stem cells and their small extracellular vesicles
Description	The heterogeneous nature of glioblastoma stem-like cells (GSCs) creates hurdles in developing effective therapies against glioblastoma, making it extremely difficult to eradicate. In this part of the project, we investigated the potential role of GSCs-derived small extracellular vesicles (sEVs) in glioblastoma heterogeneity, plasticity, and aggressiveness with the particular focus on their complexity in term of proteins. Proteome profiling of sEVs and their respective cell lines showed that GSCs-derived sEVs retain their subtype characteristics and are enriched in proteins playing a role in amino acids, carboxylic acids, organic acids transmembrane transport, and growth factor binding. In summary, we revealed the protein content of GSCs-derived sEVs and unveiled their potential contribution to tumor heterogeneity and critical cellular processes commonly deregulated in glioblastoma.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:38.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tolga Lokumcu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-27 11:36:12	ID requested
1	2024-05-21 12:22:34	announced
⏵ 2	2024-10-22 06:40:43	announced	2024-10-22: Updated project metadata.

Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, M, ü, ller G, Richter K, Poschet G, Phillips E, Goidts V, Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. ACS Nano, 18(3):2500-2519(2024) [pubmed]

10.1021/acsnano.3c11427;

submitter keyword: glioblastoma, glioblastoma stem cells, exosomes, tumor heterogeneity, proteomics,small extracellular vesicles

Dr. Violaine Goidts
contact affiliation	Brain Tumor Translational Targets B067 German Cancer Research Center (DKFZ) Im Neuenheimer Feld 580 69120 Heidelberg Germany Phone: +49 6221 42-4635
contact email	v.goidts@dkfz.de
lab head
Tolga Lokumcu
contact affiliation	German Cancer Research Center (DKFZ), Heidelberg, Germany. Faculty of Biosciences, University of Heidelberg, Heidelberg, Germany.
contact email	tolgalokumcu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036359

PRIDE project URI

• PRIDE
  1. PXD036359
     1. Label: PRIDE project
     2. Name: Proteomic profiling of glioblastoma stem cells and their small extracellular vesicles