PXD036311-1

PXD036311 is an original dataset announced via ProteomeXchange.

Title	Multisite proteome provides insights into the biology and prognostic markers for head and neck cancer
Description	The poor prognosis of head and neck cancer (HNC) is largely associated with the presence of metastasis in the lymph nodes (LN). Herein, the proteome of 140 multisite samples from a 59-HNC patient cohort, including primary and matched LN tissues, saliva, and blood cells, reveals insights into the biology and potential metastasis biomarkers that may assist in clinical decision making. The protein profiles are strictly associated with immune modulation across datasets, which provided the basis to investigate immune markers associated with metastasis. The proteome of LN metastasis cells quite recapitulates the proteome of primary sites. Conversely, the LN microenvironment proteome highlights candidate markers. By integrating selected markers at peptide, protein, and transcript levels with machine learning models, we indicate a nodal metastasis signature in blood and saliva. In summary, we present the deepest proteome characterization of multiple sampling sites in HNC, providing a promising basis to understand tumoral biology and indicating metastasis-associated biomarkers.
HostingRepository	PanoramaPublic
AnnounceDate	2022-11-22
AnnouncementXML	Submission_2022-11-22_22:28:26.951.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adriana Paes Leme
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 22:23:51	ID requested
⏵ 1	2022-11-22 22:28:27	announced

Busso-Lopes AF, Neves LX, C, â, mara GA, Granato DC, Pretti MAM, Heberle H, Patroni FMS, S, á J, Yokoo S, Rivera C, Domingues RR, Normando AGC, De Rossi T, Mello BP, Galdino NAL, Pauletti BA, Lacerda PA, Rodrigues AAN, Casarim ALM, de Lima-Souza RA, Damas II, Mariano FV, Gollob KJ, Medina TS, Cervigne NK, Prado-Ribeiro AC, Brand, ã, o TB, Villa LL, Uno M, Boroni M, Kowalski LP, Gonz, á, lez-Arriagada WA, Paes Leme AF, Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer. Nat Commun, 13(1):6725(2022) [pubmed]

submitter keyword: Head and Neck Squamous Cell Carcinoma, Mouth Neoplasms, Proteomics, Lymph Node Metastasis, Buffy coat, Saliva, Biomarkers, Prognosis, Immune System, Machine Learning

Adriana Paes Leme
contact affiliation	LNBio/CNPEM
contact email	adriana.paesleme@lnbio.cnpem.br
lab head
Adriana Paes Leme
contact affiliation	LNBio/CNPEM
contact email	adriana.paesleme@lnbio.cnpem.br
dataset submitter

Panorama Public dataset URI