PXD036289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Revealing the effect of SARS-CoV-2 spike stabilisation on receptor binding dynamics with single molecule mass photometry |
Description | Here we show using mass photometry how proline substitutions, commonly used for SARS-CoV-2 spike stabilisation and vaccine design, directly impedes ACE2 receptor interactions via dynamics of open and closed states. Conformational changes and ACE2 binding were influenced by spike variant and temperature, but independent of site-specific N-glycosylation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-28 |
AnnouncementXML | Submission_2023-06-28_03:08:38.434.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | SeanBurnap |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-25 14:27:46 | ID requested | |
⏵ 1 | 2023-06-28 03:08:38 | announced | |
2 | 2023-11-14 06:56:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: SARS-CoV-2, Glycomics, Spike, Glycoproteomics, Mass photometry, ACE2 |
Contact List
WestonStruwe |
contact affiliation | Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, OX1 3TA. |
contact email | weston.struwe@chem.ox.ac.uk |
lab head | |
SeanBurnap |
contact affiliation | Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford, UK |
contact email | sean.burnap@chem.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036289
- Label: PRIDE project
- Name: Revealing the effect of SARS-CoV-2 spike stabilisation on receptor binding dynamics with single molecule mass photometry