PXD036268-2

PXD036268 is an original dataset announced via ProteomeXchange.

Title	One-pot comparative analysis and characterization of MCF-7 and MCF-10A cell lines
Description	A comparison of different energetics based techniques for the characterization of two mammalian breast cell lines, MCF-7 a luminal A breast cancer cell line and MCF-10A a normal human breast cell line. The techniques of stability of proteins from rates of oxidation (SPROX), thermal proteome profiling (TPP), and conventional expression level analyses were compared and the relative advantages and disadvantages are discussed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:32.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Morgan Bailey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 13:15:32	ID requested
1	2023-02-22 16:51:06	announced
⏵ 2	2023-11-14 08:58:33	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Thermodynamics, thermal denaturation, chemical denaturation, protein folding

Michael C. Fitzgerald
contact affiliation	PI of the Fitzgerald lab at Duke University Depratment of Chemistry
contact email	michael.c.fitzgerald@duke.edu
lab head
Morgan Bailey
contact affiliation	Duke University Department of Chemistry
contact email	morgan.a.bailey@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036268

PRIDE project URI

• PRIDE
  1. PXD036268
     1. Label: PRIDE project
     2. Name: One-pot comparative analysis and characterization of MCF-7 and MCF-10A cell lines