PXD036258-1

PXD036258 is an original dataset announced via ProteomeXchange.

Title	mTOR activity paces human blastocyst stage developmental progression (data set 2)
Description	Many mammals can temporally uncouple conception from parturition by pacing down their development around the blastocyst stage. In mice, this dormant state is achieved by decreasing the activity of the growth-regulating mTOR signaling pathway1. It is unknown whether this ability is conserved in mammals in general and in humans in particular. Here we show that decreasing the activity of the mTOR signaling pathway induces human pluripotent stem cells (hPSCs) and blastoids to enter a dormant state with limited proliferation, developmental progression, and capacity to attach to endometrial cells. These in vitro assays show that, similar to other species, the ability to enter dormancy is active in human cells around the blastocyst stage and is reversible at both functional and molecular levels. The pacing of human blastocyst development has potential implications for reproductive therapies.
HostingRepository	PRIDE
AnnounceDate	2024-09-26
AnnouncementXML	Submission_2024-09-26_04:43:36.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 13:03:35	ID requested
⏵ 1	2024-09-26 04:43:37	announced

10.1016/J.CELL.2024.08.048;

submitter keyword: pausing embryonic development

Aydan Bulut-Karslioglu
contact affiliation	Research Group Leader Bulut-Karslioglu lab Dept. of Genome Regulation Max Planck Institute for Molecular Genetics Ihnestrasse 63-73 14195 Berlin GERMANY
contact email	Aydan.Karslioglu@molgen.mpg.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD036258

PRIDE project URI

• PRIDE
  1. PXD036258
     1. Label: PRIDE project
     2. Name: mTOR activity paces human blastocyst stage developmental progression (data set 2)