PXD036225-1

PXD036225 is an original dataset announced via ProteomeXchange.

Title	Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation
Description	Canine heartworm is a widespread and potentially fatal mosquito-borne disease caused by infections with the parasitic nematode, Dirofilaria immitis. We have previously shown that systemic activation of the Toll immune pathway via silencing of the negative regulator Cactus in Aedes aegypti blocks parasite development in the Malpighian tubules, the mosquito renal organ. However, it was not established whether the Malpighian tubules were directly responding to Toll activation or were alternatively responding to upregulated proteins or other changes to the hemolymph driven by other tissues. Distinguishing these possibilities is crucial for developing more precise strategies to block D. immitis while potentially avoiding the fitness cost to the mosquito associated with Cactus silencing. This study defines the transcriptional response of Ae. aegypti Malpighian tubules after systemic Toll activation via intra-thoracic injection of dsCactus and found, like the response of whole mosquitoes, a significant increase in expression of Toll pathway target genes. Additionally, we identified a significant overlap between the transcriptional response of the Malpighian tubules and proteins upregulated in the hemolymph. Our data show that Malpighian tubules are capable of RNAi-mediated gene silencing and directly respond to dsCactus treatment by upregulating canonical Toll pathway targets. Though not definitive, the strong correspondence between the Malpighian tubule transcriptional and the hemolymph proteomic responses provides evidence that the tubules may contribute to mosquito humoral immunity.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-27_03:43:16.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sutopa Dwivedi
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-22 03:55:44	ID requested
⏵ 1	2024-08-27 03:43:17	announced

10.1186/s13071-022-05567-2;

Sneed SD, Dwivedi SB, DiGate C, Denecke S, Povelones M, Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation. Parasit Vectors, 15(1):469(2022) [pubmed]

submitter keyword: Aedes

Hemolymph

LFQ

Cactus

Toll

Michael Povelones
contact affiliation	Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
contact email	mpove@upenn.edu
lab head
Sutopa Dwivedi
contact affiliation	School of Pathobiology, UPENN
contact email	sutopabd@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036225
     1. Label: PRIDE project
     2. Name: Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation