PXD036149-1

PXD036149 is an original dataset announced via ProteomeXchange.

Title	Structure of Saccharomyces cerevisiae Mediator-RNA polymerase II pre-initiation complex for divergent transcription
Description	This study has determined structure of transcription initiation complexes including a DNA-bound activator, RNA polymerase II (Pol II), and Mediator on a divergent promoter GAL80/SUT719 using a combination of cryo-EM and XL-MS analyses. Our cryo-EM single-particle analysis reveals a dimeric form of Med-PIC through the Mediator Tail module induced by the activator protein. Density of the upstream DNA bound to the Gal4-VP16 was identifiable along the Mediator Tail module, while XL-MS localized flexible regions that were not visible by cryo-EM analysis, such as activator-binding domains (ABDs and KIX).
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_01:15:53.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hee Jong Kim
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-18 07:56:57	ID requested
⏵ 1	2024-05-22 01:15:54	announced

10.1016/j.molcel.2023.01.011;

Gorbea Col, ó, n JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K, Structural basis of a transcription pre-initiation complex on a divergent promoter. Mol Cell, 83(4):574-588.e11(2023) [pubmed]

submitter keyword: enhancer, divergent transcription,transcription, Mediator, UAS, initiation, activator, RNA polymerase II

Kenji Murakami
contact affiliation	University of Pennsyvania
contact email	kenjim@pennmedicine.upenn.edu
lab head
Hee Jong Kim
contact affiliation	University of Pennsylvania
contact email	heejong@heejong.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036149

PRIDE project URI

• PRIDE
  1. PXD036149
     1. Label: PRIDE project
     2. Name: Structure of Saccharomyces cerevisiae Mediator-RNA polymerase II pre-initiation complex for divergent transcription